Genetic structure of Miscanthus sinensis and Miscanthus sacchariflorus in Japan indicates a gradient of bidirectional but asymmetric introgression

Clark, Lindsay V.; Stewart, J. Ryan; Nishiwaki, Aya; Toma, Yo; Kjeldsen, Jens Bonderup; Jorgensen, Uffe; Zhao, Hua; Peng, Junhua; Yoo, Ji Hye; Heo, Kweon; Yu, Chang Yeon; Yamada, Toshihiko; Sacks, Erik J.

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https://hdl.handle.net/2142/95131 Copy

Description

Title

Genetic structure of Miscanthus sinensis and Miscanthus sacchariflorus in Japan indicates a gradient of bidirectional but asymmetric introgression

Author(s)

• Clark, Lindsay V.
• Stewart, J. Ryan
• Nishiwaki, Aya
• Toma, Yo
• Kjeldsen, Jens Bonderup
• Jorgensen, Uffe
• Zhao, Hua
• Peng, Junhua
• Yoo, Ji Hye
• Heo, Kweon
• Yu, Chang Yeon
• Yamada, Toshihiko
• Sacks, Erik J.

Issue Date

2015-01-24

Keyword(s)

• Biomass crop
• cross-ploidy introgression
• hybridization
• Poaceae
• polyploidy
• population genetics
• RAD-seq

Geographic Coverage

Japan

Abstract

Unilateral introgression from diploids to tetraploids has been hypothesized to be an important evolutionary mechanism in plants. However, few examples have been definitively identified, perhaps because data of sufficient depth and breadth were difficult to obtain prior to the advent of affordable high-density genotyping. Throughout Japan, tetraploid Miscanthus sacchariflorus and diploid M. sinensis are common, and occasionally hybridize. In this study, we characterized 667 M. sinensis and 78 M. sacchariflorus genotypes from Japan using 20,704 SNPs and ten plastid microsatellites. Similarity of SNP genotypes between diploid and tetraploid M. sacchariflorus indicated that the tetraploids originated via autopolyploidy. Structure analysis indicated a gradient of introgression from diploid M. sinensis into tetraploid M. sacchariflorus throughout Japan; most tetraploids had some M. sinensis DNA. Among phenotypically M. sacchariflorus tetraploids, M. sinensis ancestry averaged 7% and ranged from 1-39%, with introgression greatest in southern Japan. Unexpectedly, rare (~1%) diploid M. sinensis individuals from northern Japan were found with 6-27% M. sacchariflorus ancestry. Population structure of M. sinensis in Japan included three groups, and was driven primarily by distance, and secondarily by geographic barriers such as mountains and straits. Miscanthus speciation is a complex and dynamic process. In contrast to limited introgression between diploid M. sacchariflorus and M. sinensis in northern China, selection for adaptation to a moderate maritime climate likely favored cross-ploidy introgressants in southern Japan. Our results will help guide the selection of Miscanthus accessions for the breeding of biomass cultivars.

Publisher

Oxford University Press

Type of Resource

• text
• dataset / spreadsheet
• image

Language

eng

Permalink

http://hdl.handle.net/2142/95131

DOI

https://doi.org/10.1093/jxb/eru511

Sponsor(s)/Grant Number(s)

• Office of Science - Biological and Environmental Research, U.S. Department of Energy [Project ID 0017582]
• Energy Biosciences Institute

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