Raman spectroscopic analysis of fatty acids in tumor micro- and macroenvironments in breast cancer

You, Sixian

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Description

Title

Raman spectroscopic analysis of fatty acids in tumor micro- and macroenvironments in breast cancer

Author(s)

You, Sixian

Issue Date

2015-12-07

Director of Research (if dissertation) or Advisor (if thesis)

Boppart, Stephen A.

Department of Study

Bioengineering

Discipline

Bioengineering

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

M.S.

Degree Level

Thesis

Keyword(s)

• Raman spectroscopy
• Breast cancer

Abstract

Fatty acids play essential roles in the growth and metastasis of cancer cells. To facilitate their avid growth and proliferation, cancer cells not only alter the fatty acid synthesis and metabolism intracellularly and extracellularly, but also in the macroenvironment via direct and indirect pathways. This thesis reports that by using Raman micro-spectroscopy, an increase in the production of polyunsaturated fatty acids (PUFAs) was identified in both cancerous and normal appearing breast tissue obtained from breast cancer patients and tumor-bearing rats. By minimizing confounding effects from mixed chemicals and optimizing the signal-to-noise ratio of Raman spectra, a large-scale transition from monounsaturated fatty acids to PUFAs was observed in the tumor while only a small subset of fatty acids transitioned to PUFAs in the tumor micro- and macroenvironment. These findings demonstrate the power of this spectroscopic analysis, and may provide new insights into the macroenvironmental regulations in breast cancer.

Graduation Semester

2016-05

Type of Resource

text

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http://hdl.handle.net/2142/90452

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Copy right 2015 Sixian You

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