University of Illinois Urbana-Champaign

Investigating the role of Extracellular Matrix (ECM) remodeling during wing disc regeneration in Drosophila

Sul, Amanda

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125630.pdf

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https://hdl.handle.net/2142/90255

Description

Title
Investigating the role of Extracellular Matrix (ECM) remodeling during wing disc regeneration in Drosophila
Author(s)
Sul, Amanda
Contributor(s)
Smith-Bolton, Rachel
Issue Date
2016
Keyword(s)
  • Drosophila
  • Regeneration
  • Basement Membrane
  • Extracellular Matrix
  • Mmp
Date of Ingest
2016-06-09T19:14:01Z
Abstract
Some animals are capable of regenerating parts of their body after damage, whereas other animals are only capable of wound healing and scarring. Tissue regeneration is the replacement of damaged tissue and restoration of morphology as well as functionality. Regeneration requires a complex interplay between extracellular matrix (ECM) and cells within a tissue. Matrix metalloproteinases (MMPs) are responsible for degrading the ECM and are highly expressed at wound sites. However, the exact role of the ECM and MMPs in the wound healing and regenerative process is not yet clear. I employed a non-surgical method of inducing tissue damage and regeneration in the wing imaginal disc of Drosophila, which is highly amenable to genetic manipulations. Using this system I tracked the expression of Viking (Vkg), the gene coding for ECM component Collagen IV, during different stages of regeneration after tissue damage. I observed disruptions in the basement membrane structure as assayed by the Green Fluorescent Protein (GFP) tagged reporter Vkg-GFP. I also observed changes in MMP protein expression levels during various stages of regeneration. I further investigated the effects of overexpressing and inhibiting MMP expression to understand the functional significance of MMP in the regenerating wing discs. I observed decreased wing regeneration with overexpression of MMP. I have also examined the role of Jun N terminal Kinase (JNK) signaling in ECM remodeling during regeneration.
Type of Resource
text
Genre of Resource
Conference Poster
Language
en
Permalink
http://hdl.handle.net/2142/90255
Copyright and License Information
Copyright 2016 Amanda Sul

Owning Collections

Undergraduate Research Symposium 2016 PRIMARY