Developing a Genomic Protocol for Identification of Tick-borne Pathogens in Laikipia, Kenya
Lincoln, Katherine D.
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https://hdl.handle.net/2142/90236
Description
Title
Developing a Genomic Protocol for Identification of Tick-borne Pathogens in Laikipia, Kenya
Author(s)
Lincoln, Katherine D.
Contributor(s)
Allan, Brian
Issue Date
2016
Keyword(s)
Fluidigm
next-generation sequencing
ticks
tick-borne disease
Kenya
Abstract
The region of Laikipia, Kenya, is home to a diversity of wild and domestic mammal hosts for Ixodid ticks. These tick species act as vectors for numerous infectious diseases, including economically important diseases such as East Coast fever.'Due to the effects of tick-borne disease on livestock, cattle in Kenya are treated with acaricides, which reduce tick loads and may also reduce disease for wildlife. Using field-collected adult and nymphal life-stage ticks, I extracted and analyzed pathogen DNA. I developed several functional primer sets for Fluidigm, a microfluidic process consisting of polymerase chain reaction (PCR) analysis followed by MiSeq. PCR products were analyzed for pathogen identity with BLAST and number of sequence reads. The development of this protocol will help to identify and combat tick-borne pathogens around the world.
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