Putative regulatory role of GlyS antisense RNA in an obligate insect symbiont Buchnera aphidicola

Cheng, Siyuan

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https://hdl.handle.net/2142/90105 Copy

Description

Title

Putative regulatory role of GlyS antisense RNA in an obligate insect symbiont Buchnera aphidicola

Author(s)

Cheng, Siyuan

Contributor(s)

Hansen, Allison K.

Issue Date

2016

Keyword(s)

small RNA, obligate insect symbiont, gene regulation, bacteria, Buchnera aphidicola

Abstract

My research seeks to answer the question of how small RNAs regulate the gene expression in an uncultivable obligate insect symbiont Buchnera aphidicola, which is important for deeper understanding of the influence of gene regulation on host-symbiont interaction and co-evolution. This presentation will discuss how I apply the novel dual plasmid vector system to the investigation of an uncultivable symbiont gene regulation in vivo. Thus far, the plasmids encoding the antisense RNA (asRNA) of a candidate gene (glyS) has been constructed and transformed into E. coli cells. Next, the DNA coding sequence (CDS) of glyS will be amplified and restrict-digested. This will enable the other plasmids to be constructed with the CDS and transformed into E. coli cells. The activation or inhibition of the gene expression by the asRNA will be measured with the green fluorescent protein (GFP) that is fused with the CDS. The research would lead to more insights on how small RNAs regulate the gene expression of bacteria with reduced genome in the absence of transcription factors and operons. These insights would help us understand the mechanisms of gene regulation in bacteria, which would decipher the genome co-evolution of hosts and symbionts.

Type of Resource

text

Language

en

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http://hdl.handle.net/2142/90105

Copyright and License Information

Copyright 2016, Cheng

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