The coaggregative abilities of lactobacilli and Campylobacter jejuni

Wooten, Jessica Michelle

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https://hdl.handle.net/2142/89236 Copy

Description

Title

The coaggregative abilities of lactobacilli and Campylobacter jejuni

Author(s)

Wooten, Jessica Michelle

Issue Date

2015-12-10

Director of Research (if dissertation) or Advisor (if thesis)

Miller, Michael J.

Department of Study

Food Science & Human Nutrition

Discipline

Food Science & Human Nutrition

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

M.S.

Degree Level

Thesis

Keyword(s)

• Lactobacillus
• Campylobacter jejuni
• coaggregation

Abstract

Campylobacter jejuni is a pathogen commonly found in poultry that is one of the main bacterial causes of foodborne diarrheal disease in humans. The aim of this study was to analyze the coaggregative abilities of lactic acid bacteria and C. jejuni as a means to reduce the colonization of C. jejuni in poultry. In this study, various lactic acid bacteria were screened to analyze their coaggregative abilities with C. jejuni. The coaggregative abilities of LAB strains within the same species were compared. L. crispatus’ ability to coaggregate with C. jejuni mutant strains was tested. The generation of non-coaggregating L.crispatus variants was performed. DNA sequencing was performed on the wildtype strains of L.crispatus (MJM 207 and 207) along with three non-coaggregating variants. All of these measures were used to help pinpoint a genetic determinant for the coaggregative ability of the LAB strains tested. L.plantarum, L. acidophilus, and L. fermentum did not display significant variation of coaggregative abilities with C.jejuni. L. crispatus was not able to coaggregate with C.jejuni mutant strains (MJM 376, 378, 379, 381 and 382). Findings from this study can provide knowledge to help better understanding the coaggregative relationship between lactobacilli and C. jejuni.

Graduation Semester

2015-12

Type of Resource

text

Permalink

http://hdl.handle.net/2142/89236

Copyright and License Information

Copyright 2015 Jessica Wooten

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