University of Illinois Urbana-Champaign

Micron-bubble streaming flow towards micron to sub-micron sized sorting

Yang, Rui

Content Files
YANG-THESIS-2015.pdf

Permalink

https://hdl.handle.net/2142/89091

Description

Title
Micron-bubble streaming flow towards micron to sub-micron sized sorting
Author(s)
Yang, Rui
Issue Date
2015-12-10
Director of Research (if dissertation) or Advisor (if thesis)
Hilgenfeldt, Sascha
Department of Study
Mechanical Science & Engineering
Discipline
Mechanical Engineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Date of Ingest
2016-03-02T19:45:14Z
Keyword(s)
  • Microfluidics
  • sorting
  • micron-bubble
Abstract
Micro-bubble streaming flows represent a unique type of actuating mechanism for microfluidics and have demonstrated great potential in the applications of micro-particle manipulation (e.g. size dependent trapping, sorting, and focusing etc.). The main object of this thesis work is to extend towards much smaller sized particle sorting down to 1μm and sub-micron. On top of that, we want to further explore the potential of applying bubble streaming sorting to biological objects which in the similar size scales. Systematical experiments have been conducted on polystyrene latex particles as well as several types biological cells and its infectious viruses, specifically Sulfolobus islandicus with Sulfolobus spindle-shaped viruses, and also Escherichia coli with Lambda phage. Size sensitive sorting has been revealed by means of larger sized objects will be majorly deflected away from their original streamline while smaller sized objects will follow, which leads to size-sensitive sorting. It has been proved that relative concentration ratio of two sized particle mixing solution can be enhanced with almost a factor of 6, and can be further improved by sequential bubble sorting. However, sorting application with smaller particles with diameter as 1μm and 100nm doesn’t perform as well as larger particles sorting reported by Wang et al. (Appl. Phys. Lett. 99, 034101, 2011) when they used a mixture of 10μm and 5μm particles in diameter. Fundamental reason of this can be explained by a combination of advection and diffusion effect. Beyond that, the principle of micro-bubble streaming sorting also has shown applicable to biological objects sorting purpose, but could be affected more by (i) wider size distribution, (ii) naturally morphologically diverse, (iii) active motion of live cells and (iv) more deformable compared to artificial polystyrene particles.
Graduation Semester
2015-12
Type of Resource
text
Permalink
http://hdl.handle.net/2142/89091
Copyright and License Information
Copyright 2015 Rui Yang

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Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Mechanical Science and Engineering