The heterogeneous biochemical modification of porous collagen scaffolds for tissue engineering applications

Turgeon, Aurora

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https://hdl.handle.net/2142/87973 Copy

Description

Title

The heterogeneous biochemical modification of porous collagen scaffolds for tissue engineering applications

Author(s)

Turgeon, Aurora

Issue Date

2015-07-06

Director of Research (if dissertation) or Advisor (if thesis)

Bailey, Ryan C.

Doctoral Committee Chair(s)

Bailey, Ryan C.

Committee Member(s)

• Harley, Brendan A.
• Murphy, Catherine J.
• Perry, Richard H.

Department of Study

Chemistry

Discipline

Chemistry

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

• Photopatterning
• benzophenone
• scaffold

Abstract

The extracellular matrix is a complex and dynamic collection of collagens, non-collagenous matrix proteins, glycosaminoglycans and growth factors. The ECM plays a significant role in guiding the behavior of cells in their tissue specific niche. The recapitulation of both the mechanical and biochemical cues of the ECM is an important objective towards in vitro engineering of functional tissue replacements. Herein I describe my efforts towards the biochemical modification of 3D, porous collagen scaffolds as ECM mimics. Benzophenone mediated photochemistry was used to decorate scaffolds with patterns of vascular endothelial growth factor, which were shown to induce endothelial cells towards a vasculogenic lineage. The use of a two photon excitation for BP patterning was proven affective for surface patterning but ineffective for patterning past a couple hundreds of microns. Heterogenous scaffold patterning in all three dimensions was achieved by collagen slurry layering prior to scaffold freeze drying. A single scaffold was created with two distinct compartment a high modulus “bone” compartment and a low modulus “cartilage” compartment. . This technique was used to model the osteochondral interface.

Graduation Semester

2015-8

Type of Resource

text

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http://hdl.handle.net/2142/87973

Copyright and License Information

Copyright 2015 Aurora Turgeon

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