University of Illinois Urbana-Champaign

From Technology to Chromatin Biology: Contemporary Mass Spectrometry of Histones and Chromatin Associated Proteins

Siuti, Nertila

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Description

Title
From Technology to Chromatin Biology: Contemporary Mass Spectrometry of Histones and Chromatin Associated Proteins
Author(s)
Siuti, Nertila
Issue Date
2008
Doctoral Committee Chair(s)
Kelleher, Neil L.
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
We have also developed a top down-like approach (middle down) which analyzes large peptides to characterize PTM profiles on histone H3. Interestingly, we were able to conclude that methylation and acetylation levels on histone H3 are highly effected by perturbations of DNA methylation. In the past years we have expanded the applications of top down and middle down MS to other chromatin-associated proteins like HMGA1. In this study we were able to link PTM dynamics (phosphorylation of Ser 43) on HMGA1a to DNA repair and the early events in apoptosis.
Graduation Semester
2008
Type of Resource
text
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http://hdl.handle.net/2142/87897

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