Development of Functional DNA -Based Sensors and Investigations Into Their Mechanism
Nagraj, Nandini
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https://hdl.handle.net/2142/87892
Description
Title
Development of Functional DNA -Based Sensors and Investigations Into Their Mechanism
Author(s)
Nagraj, Nandini
Issue Date
2010
Doctoral Committee Chair(s)
Lu, Yi
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Inorganic
Language
eng
Abstract
The starting point towards the development of a successful functional nucleic acid-based sensor is its isolation and this is done through in vitro selection. In vitro selection to isolate DNAzymes for Hg2+ and Cd2+ and the use of negative selections to overcome Pb 2+ interference at various stages of selection has been described in Chapter 5. Chapter 6 describes the structure-switching strategy to isolate DNA aptamers specific for endotoxins. It is anticipated that the results obtained from the current study and future characterizations will lead to the development of functional DNA sensors for endotoxins.
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