Manipulation of Anthocyanin Accumulation by Vaccinium Pahalae in Vitro

Fang, Yimin

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https://hdl.handle.net/2142/87830 Copy

Description

Title

Manipulation of Anthocyanin Accumulation by Vaccinium Pahalae in Vitro

Author(s)

Fang, Yimin

Issue Date

1999

Doctoral Committee Chair(s)

Mary Ann L. Smith

Department of Study

Horticulture

Discipline

Horticulture

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

Biology, Cell

Language

eng

Abstract

Elicitation of the cells by both biotic (purified beta-glucan and chitosan) and abiotic (FeEDDHA and CuSO4) elicitors resulted in significant enhancement of anthocyanin accumulation. Maximum anthocyanin production using the two most effective elicitors (90 muM FeEDDHA or 10 mg L -1 beta-glucan) was achieved when cultures were treated on day 3 (beta-glucan) or day 0 (FeEDDHA) after the initiation of the cultures. A concentration-dependent response was exhibited by cultures treated with exogenous methyl jasmonate (MJ), a signaling compound. The addition of 0.5 muM MJ alone provoked a striking 2.8 fold increase in anthocyanin production over the control, however, no additive effect on anthocyanin production was observed in any treatments which combined MJ and beta-glucan or FeEDDHA. Conditioning of the cells with a preculture in either MJ, beta-glucan or FeEDDHA similarly did not enhance anthocyanin production. Inoculation of cultures elicited by MJ or beta-glucan with ibuprofen, a reported inhibitor of jasmonate biosynthesis, dramatically stimulated, rather than inhibited, anthocyanin production, resulting in levels of accumulation beyond any of the tested elicitor combinations.

Graduation Semester

1999

Type of Resource

text

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http://hdl.handle.net/2142/87830

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