Applied Biotechnology for in Vitro Production of Carica Papaya L

Castillo, Bernarda Altagracia

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Description

Title

Applied Biotechnology for in Vitro Production of Carica Papaya L

Author(s)

Castillo, Bernarda Altagracia

Issue Date

1997

Doctoral Committee Chair(s)

Smith, Mary Ann Lila

Department of Study

Horticulture

Discipline

Horticulture

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

Biology, Plant Physiology

Language

eng

Abstract

A suitable in vitro system for the production of Carica papaya L. was developed. Shoot cultures of papaya were optimally established at low irradiance, 5 $\mu$mol m$\rm\sp{-2}s\sp{-1},$ with FeEDTA as the iron source. But during later proliferation, highest multiplication rates, highest leaf chlorophyll concentrations, and significantly improved shoot quality were achieved at 30 $\mu$mol m$\rm\sp{-2}s\sp{-1}$ irradiance when two iron chelate sources, FeEDTA and FeEDDHA, were combined (each at a 100 $\mu$M concentration) in the medium. A higher frequency of somatic embryogenesis was achieved in a shorter time in a liquid system than reported previously for other systems. The presence of abscisic acid (ABA) in the maturation medium enhanced embryo size and increased the percentage developing into plantlets. A papain-like enzyme was detected in the somatic embryo cultures both under light or in darkness, but enzyme activity was enhanced in a liquid system under 40$\mu$mol m$\rm\sp{-2}s\sp{-1}$ irradiance. Artificial seeds were developed by encapsulation of somatic embryos. The exposure of the beads to calcium chloride for 10 minutes was found to be optimal for higher conversion frequency into plantlets.

Graduation Semester

1997

Type of Resource

text

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