Applications of RAPD Markers in Soybean: Genetic Diversity and Linkage Analysis
Li, Zenglu
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https://hdl.handle.net/2142/87684
Description
Title
Applications of RAPD Markers in Soybean: Genetic Diversity and Linkage Analysis
Author(s)
Li, Zenglu
Issue Date
1999
Doctoral Committee Chair(s)
Nelson, Randall L.
Department of Study
Agronomy
Discipline
Agronomy
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
To determine the efficacy of the pollen tube pathway transformation procedure plasmids carrying the bar and gus genes were applied to cut stigmas. Approximately 5000 seeds were produced from treated flowers. No plants derived from seeds produced by flowers treated with the plasmid carrying the bar gene were found to be as herbicide resistant as the positive control. Approximately 2% of seeds treated with the gus gene had a positive GUS reaction but primers specific for the gus gene failed to detect the gene.
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