Non-Invasive Detection of Anaerobic Metabolism in Exercising Muscle Using Proton MRI
Damon, Bruce Murray
This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.
Permalink
https://hdl.handle.net/2142/87273
Description
Title
Non-Invasive Detection of Anaerobic Metabolism in Exercising Muscle Using Proton MRI
Author(s)
Damon, Bruce Murray
Issue Date
2000
Doctoral Committee Chair(s)
Dawson, M. Joan
Department of Study
Molecular and Integrative Physiology
Discipline
Molecular and Integrative Physiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Health Sciences, Radiology
Language
eng
Abstract
The second, and major, component of this thesis concerned the exercise-induced increases the 1H T2 of muscle water. The hypotheses were tested that the T2 increase results from increased intracellular volume (VI), decreased intracellular pH (pHI), and/or changes in magnetization transfer (MT) or other longitudinal relaxation mechanisms. Isolated frog gastrocnemii were treated with iodoacetate (IAA; to inhibit lactate production), NaCN (to maximize lactate production), or left untreated and then performed 60 1s/min maximal isometric contractions. The intracellular transverse relaxation time (T2,I increased more in NaCN-treated and untreated muscles than in IAA-treated muscles. The VI increase and pHI decrease were greater in NaCN-treated and untreated muscles than in IAA-treated muscles. There was no change in the MT ratio and a slight decrease in T1 following exercise. In separate experiments, V I and pHI were manipulated in non-exercising sartorii and quantitative relationships were developed between these variables and T 2,I. A multiple regression model shows that 62% of the variance in the T2,I change of exercise is accounted for by these two variables.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
