Calcium Influx via the T-Type Calcium Channel Plays a Permissive Role in Proliferation of Mouse Embryonic Hl-1 Cells

Janes, Donna Marie

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Description

Title

Calcium Influx via the T-Type Calcium Channel Plays a Permissive Role in Proliferation of Mouse Embryonic Hl-1 Cells

Author(s)

Janes, Donna Marie

Issue Date

2004

Doctoral Committee Chair(s)

Philip Best

Department of Study

Molecular and Integrative Physiology

Discipline

Molecular and Integrative Physiology

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

Biology, Cell

Language

eng

Abstract

Cardiac myocytes express two types of voltage operated calcium currents, a high voltage activated (HVA) L-type, and a low voltage activated (LVA) T-type. Influx of calcium into the cell through the L-type channel is responsible for excitation-contraction coupling in the heart. The T-type calcium current has been associated with growth and differentiation in a number of different cell types. An atrial myocyte cell line (HL-1) that selectively expresses T-type calcium current was employed to show that inhibiting calcium influx through the T-type calcium channel inhibits cellular proliferation. Drug dosage studies demonstrate that the T-type calcium channel responsible for this effect is Cav 3.1. Furthermore, blocking the calcium influx through the T-type calcium channel arrests cells in the G2/M phase of the cell cycle. Interestingly, the proliferative effect of calcium influx through the T-type calcium channel appears to happen in the early G1 phase of the cell cycle.

Graduation Semester

2004

Type of Resource

text

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http://hdl.handle.net/2142/87230

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