Cholinergic Regulation of Circadian Rhythms in the SCN: Characterization of Signaling Mechanisms and the Role of Intracellular Calcium in the Directionality of Clock Resetting
Artinian, Liana
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https://hdl.handle.net/2142/87212
Description
Title
Cholinergic Regulation of Circadian Rhythms in the SCN: Characterization of Signaling Mechanisms and the Role of Intracellular Calcium in the Directionality of Clock Resetting
Author(s)
Artinian, Liana
Issue Date
2001
Doctoral Committee Chair(s)
Gillette, Martha U.
Department of Study
Molecular and Integrative Physiology
Discipline
Molecular and Integrative Physiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Neuroscience
Language
eng
Abstract
This study shows that stimulus-specific intracellular Ca2+ (Ca2+i) release controls the direction of clock resetting. Direct activation of the InsP3-induced Ca2+ i release (IICR) mimics cholinergic phase advance, while InsP3 , but not ryanodine, receptor antagonists block it. In contrast, selective activation of CICR with ryanodine results in glutamate-like delay. Ca 2+ imaging using multiphoton microscopy revealed signal-specific differences in Ca2+ transients. Cholinergic-stimulated Ca2+ transients are localized to the microdomains in the cytoplasm, whereas glutamate-induced Ca2+ transients are predominantly uniform across the cytosol and nucleus. Thus, the direction of clock resetting is determined by differential Ca2+i release that is likely to result in activation of spatially restricted Ca2+-dependent effector proteins.
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