Regulation of Intracellular pH and Plasma Membrane Potential in Trypanosoma Cruzi and Trypanosoma Brucei
VanDerHeyden, Nicole M.J.
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Permalink
https://hdl.handle.net/2142/87146
Description
Title
Regulation of Intracellular pH and Plasma Membrane Potential in Trypanosoma Cruzi and Trypanosoma Brucei
Author(s)
VanDerHeyden, Nicole M.J.
Issue Date
2000
Doctoral Committee Chair(s)
Roberto Docampo
Department of Study
Veterinary Clinical Medicine
Discipline
Veterinary Clinical Medicine
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Regulation of intracellular pH (pHi) and the plasma membrane potential (DeltaPsi) was investigate in all stages of the pathogenic protozoans, T. cruzi and T. brucei utilizing the pH sensitive fluorescent dye BCECF and the potentiometric fluorescent dye bisoxonol. pH i in T. cruzi was found to be regulated primarily by a H+-ATPase with support from a Cl- conductive channel in all stages and an inwardly directed K+ channel in the mammalian stages. The DeltaPsi in T. cruzi was found to be generated by a H+-ATPase in all stages however there were significant differences in its regulation between the different stages. The trypomastigote (mammalian bloodstream) stage was found to be highly conductive to cations and there was a significant contribution to its DeltaPsi from a ouabain sensitive Na+/K+-ATPase. The epimastigote or insect stage was found to have a low conductance for cations and a minor contribution from the Na+/K+-ATPase. There was no evidence for cation conductance or a Na+/K+-ATPase in the intracellular mammalian amastigote stage. pHi in T. brucei procyclic or insect stages was found to be regulated primarily by a H+-ATPase with support from a Cl- conductive channel. In the mammalian bloodstream trypomastigote for pH i was regulated by a pyruvate-proton symport with a minor contribution from a H+-ATPase and supported by a Cl- conductive channel. The DeltaPsi of both procyclic and bloodstream forms was generated by a H+-ATPase. The bloodstream stage was found to be highly conductive to cations and there was a significant contribution to its DeltaPsi from a ouabain sensitive Na+/K+ -ATPase. The procyclic or insect stage was found to have a low conductance for cations and a minor contribution from the Na+/K +-ATPase.
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