Biochemical and Molecular Characterizations of a Novel DNA Repair Enzyme, CPD -Photolyase, and Late Promoters of Fowlpox Virus
Srinivasan, Viswanathan
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https://hdl.handle.net/2142/87118
Description
Title
Biochemical and Molecular Characterizations of a Novel DNA Repair Enzyme, CPD -Photolyase, and Late Promoters of Fowlpox Virus
Author(s)
Srinivasan, Viswanathan
Issue Date
2002
Doctoral Committee Chair(s)
Deoki N. Tripathy
Department of Study
Veterinary Clinical Medicine
Discipline
Veterinary Clinical Medicine
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Health Sciences, Immunology
Language
eng
Abstract
To further exploit FPV as a vector, characterization of strong promoters were attempted using a lacZ gene based reporter assay. The transcriptional element of ORF P190 appeared to be the strongest. In contrast, the promoter associated with the ORF, P180, was the weakest. Interestingly, a novel bi-directional promoter controlling the expression of ORFs 130 and 131 appeared to be active whose expression was one log fold less than that of the P190 promoter. Subsequently when the critical positions in the spacer and the thymidine after the start codon were substituted with those present in P190, the activity of the weakest promoter was increased to as high as thirty times more than the unaltered promoter, P180. When similar substitutions were made in the strongest promoter, P190, its activity was reduced to 50%. Thus the present study underscores the significance of nucleotides flanking the transcriptional and translational start sites. The promoters identified from this study, P190, P191, P174, as well as the genetically manipulated promoter, P180SG and the bi-directional promoter, P130 and P131 can be used for the expression of foreign genes by FPV vectors.
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