Molecular Cloning and Structure-Function Analysis of the Plant Proton-Sucrose Symporter
Lu, Mei-Yeh Jade
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https://hdl.handle.net/2142/87067
Description
Title
Molecular Cloning and Structure-Function Analysis of the Plant Proton-Sucrose Symporter
Author(s)
Lu, Mei-Yeh Jade
Issue Date
1998
Doctoral Committee Chair(s)
Bush, Daniel R.
Department of Study
Plant Biology
Discipline
Plant Biology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Cell
Language
eng
Abstract
"To explore other functionally-important domains, the AtSUC1 cDNA was randomly mutagenized and transformed yeast were screened on sucrose- or maltose-limited media for growth. Three point mutations were identified---H65Q, G334S, and L461P. Significantly, they all displayed enhanced sucrose transport rate. H65Q and G334S also increased maltose transport activity. In the predicted ""6-loop-6"" topology of the symporter, these residues all face extracellularly, suggesting their involvement in substrate binding. To investigate this hypothesis, the symporter-expressing COS-1 cells were analyzed by epitope-tagging and immunofluorescence. The results showed that both N- and C-termini, as well as the central loop, are located on the cytoplasmic side of the membrane."
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