Characterization and Functional Analysis of the Arabidopsis Intron Splicing Factor ASF/SF2
Su, Chun-Lin
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https://hdl.handle.net/2142/87064
Description
Title
Characterization and Functional Analysis of the Arabidopsis Intron Splicing Factor ASF/SF2
Author(s)
Su, Chun-Lin
Issue Date
1998
Doctoral Committee Chair(s)
Schuler, Mary A.
Department of Study
Plant Biology
Discipline
Plant Biology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
ASF/SF2 protein is first time shown to be a potential substrate for the mitotic cdc2/cyclin kinase. UV crosslinkage assay demonstrate RNA binding ability of SF2a and RRM domain, but not SF2b protein. Immunostaining of TN-5 cells expressing recombinant proteins and western blot analyzing cytoplasmic and nuclear fractions of expression cells showed targeting of SF2a, SF2b and their SR domains to the nuclei. SR domain, possibly required phosphorylation, is responsible for the nuclear localization signal.
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