Identifying the Rubisco Recognition Domain and Critical Arginines in Activase and Engineering Activase With Improved Thermostability for Arabidopsis
Li, Cishan
This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.
Permalink
https://hdl.handle.net/2142/87049
Description
Title
Identifying the Rubisco Recognition Domain and Critical Arginines in Activase and Engineering Activase With Improved Thermostability for Arabidopsis
Author(s)
Li, Cishan
Issue Date
2006
Doctoral Committee Chair(s)
Archie R. Portis, Jr.
Department of Study
Plant Biology
Discipline
Plant Biology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Heat sensitivity of Rubisco activase(s) confers a major limitation on leaf photosynthesis at moderately high temperature in Arabidopsis. To overcome this limitation, a thermostable activase for Arabidopsis was generated by engineering the more thermostable tobacco activase with an Arabidopsis Rubisco recognition domain. The resulting chimeric activase displayed an improved thermostability, activated Arabidopsis Rubisco and maintained Rubisco activation state at moderately high temperature in vitro. Transgenic Arabidopsis plants expressing the chimeric activase were created, allowing for further characterization.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
