University of Illinois Urbana-Champaign

Effects of Changes of Redox Properties of the Chloroplast ATP Synthase in Arabidopsis

Wu, Guosheng

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Description

Title
Effects of Changes of Redox Properties of the Chloroplast ATP Synthase in Arabidopsis
Author(s)
Wu, Guosheng
Issue Date
2003
Doctoral Committee Chair(s)
Ort, Donald R.
Department of Study
Plant Biology
Discipline
Plant Biology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Plant Physiology
Language
eng
Abstract
"Three mutations, designated as C199S, C205S, C199S/C205S, were generated by transformating wild type (Columbia ecotype) plants with the site-directed mutated gene constructs of gamma-subunit. The transgenes were overexpressed behind a constitutive 35S promoter compared with the endogenous gamma-subunit gene. In the isolated mutant chloroplasts, the high ATPase activities did not require DTT treatment while in the wild-type plant, ATPase activities increased after DTT treatment indicating that the cysteine to serine substitutions caused loss of the redox regulation and the ATP synthases were locked in reduced configuration. The ""permanently reduced"" ATP synthases could not be fully deactivated in the dark. There was no significant difference in photosynthesis rate and growth rate suggesting the redox regulation is not very important for plants."
Graduation Semester
2003
Type of Resource
text
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http://hdl.handle.net/2142/87037

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