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https://hdl.handle.net/2142/86768
Description
Title
Directed Evolution of T Cell Receptor Antagonists
Author(s)
Kieke, Michele Catherine
Issue Date
2001
Doctoral Committee Chair(s)
Kranz, David M.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
In Chapter 4, further engineering of TCR stability mutants to produce increased affinity binding for SAg is described. An optimized TCR fragment (containing nine mutations) was isolated after mutagenesis and selection, and was found to have approximately 1000-fold improved affinity for SAg. A soluble form of the mutant was shown to completely and specifically inhibit T cell activity in vitro.
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