University of Illinois Urbana-Champaign

Angiotensin II Modulation of NMDA Receptor Function: Characterization of Mechanisms Attenuating Excitotoxicity in Neurons

Schelman, William Russell

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Description

Title
Angiotensin II Modulation of NMDA Receptor Function: Characterization of Mechanisms Attenuating Excitotoxicity in Neurons
Author(s)
Schelman, William Russell
Issue Date
2000
Doctoral Committee Chair(s)
Weyhenmeyer, James A.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
In the last study, we investigated the involvement of Bcl-2 in the suppression of NMDA-mediated neurotoxicity by Ang II. The viability of differentiated N1E and NG108 neuronal cell lines was reduced following exposure to NMDA in a dose-dependent manner. Excitotoxicity in N1E cells was inhibited by the noncompetitive NMDA receptor antagonist, MK-801. Further, NMDA receptor-mediated cell death was attenuated by Ang II in NG108 cells. A concentration-dependent decrease in intracellular Bcl-2 protein levels was observed following treatment with NMDA, and this reduction was inhibited by MK801. Addition of Ang II suppressed the NMDA receptor-mediated reduction in Bcl-2. The Ang II effect on NMDA-mediated changes in Bcl-2 expression was blocked by PD 123319, but was not significantly altered by losartan. Taken together, these results suggest that the decrease in Bcl-2 expression induced by NMDA is attenuated by AT2 activation. (Abstract shortened by UMI.).
Graduation Semester
2000
Type of Resource
text
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http://hdl.handle.net/2142/86759

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