University of Illinois Urbana-Champaign

Signals Regulating Organelle Transport in Melanophores

Reilein, Amy Ruth

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https://hdl.handle.net/2142/86758

Description

Title
Signals Regulating Organelle Transport in Melanophores
Author(s)
Reilein, Amy Ruth
Issue Date
2000
Doctoral Committee Chair(s)
Vladimir I. Gelfand
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Cell
Language
eng
Abstract
To investigate the mechanisms of regulation of motor proteins driving melanosome transport, the phosphorylation states of the motors and the levels of motors on the organelles were examined. Immunoprecipitation of myosin V showed that the motor subunit is more heavily phosphorylated in cells aggregating pigment compared to cells dispersing pigment. Furthermore, levels of myosin V on melanosomes are higher in cells dispersing pigment. Therefore the association of myosin V with melanosomes correlates with its dephosphorylation. Immunoprecipitation of cytoplasmic dynein showed that proteins at the molecular weights of the light intermediate chains are differentially phosphorylated in aggregated and dispersed cells. Both motor subunits of kinesin II are phosphorylated in melanophores, but were not observed to be differentially phosphorylated. The levels of kinesin II and dynein on aggregating or dispersing melanosomes remain constant.
Graduation Semester
2000
Type of Resource
text
Permalink
http://hdl.handle.net/2142/86758

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Dissertations and Theses - Microbiology