Functional Analysis of the N -Terminal Transmembrane/signal -Anchor Sequence and Linker Domain of Cytochrome P450 2C2
D., Balraj
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https://hdl.handle.net/2142/86753
Description
Title
Functional Analysis of the N -Terminal Transmembrane/signal -Anchor Sequence and Linker Domain of Cytochrome P450 2C2
Author(s)
D., Balraj
Issue Date
2000
Doctoral Committee Chair(s)
Kemper, Byron W.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
Truncated forms of P450 2C2 lacking the N-terminal hydrophobic signal anchor sequence (Delta2- 20) or the signal anchor and linker region (Delta2-27) were expressed alone or as GFP fusions in E. coli and insect cells. The findings indicate that deletion of the signal anchor decreases assembly of P450 hemoprotein in E. coli but does not change the in vivo distribution of the protein. However, it alters the nature of membrane interaction such that P450 2C2 is no longer an integral membrane protein. Moreover, the results show that the linker domain is critical for the formation of functional P450 2C2 only when the P450 molecule is properly targeted and inserted in the membrane.
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