University of Illinois Urbana-Champaign

Molecular, Genetic and Physiological Studies on the Regulation of Conjugal Transfer of the Ti Plasmid pTiC58 by Agrobacterium Tumefaciens

Piper, Kevin Ray

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Description

Title
Molecular, Genetic and Physiological Studies on the Regulation of Conjugal Transfer of the Ti Plasmid pTiC58 by Agrobacterium Tumefaciens
Author(s)
Piper, Kevin Ray
Issue Date
1999
Doctoral Committee Chair(s)
Farrand, Stephen K.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
Conjugation assays determined that the bacteria mate in a cell density dependent manner, known as quorum-sensing. Analysis of transfer frequencies using a transfer-constitutive mutant of pTiC58 showed that quorum sensing requires both the opine induction and autoinduction systems. Analysis of lacZ fusions determined that quorum-sensing results from TraR and AAI activating transfer gene expression only at high cell densities. The bacteria measure their density by sensing the concentration of AAI in the growth medium.
Graduation Semester
1999
Type of Resource
text
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http://hdl.handle.net/2142/86750

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Dissertations and Theses - Microbiology