University of Illinois Urbana-Champaign

Molecular and Genetic Characterization of SAS Genes in Position Effect Silencing in Saccharomyces Cerevisiae

Xu, Eugenia Yujin

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Description

Title
Molecular and Genetic Characterization of SAS Genes in Position Effect Silencing in Saccharomyces Cerevisiae
Author(s)
Xu, Eugenia Yujin
Issue Date
1998
Doctoral Committee Chair(s)
David H. Rivier
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
Further characterization of SAS4 and SAS5 demonstrates that they define a new SIR-dependent silencing pathway in yeast. SAS4 and SAS5 are required for silencing at teloments while SIR1 is not. Also, SAS4 and SAS5 have opposite phenotype from SIR1 at HMR. These results indicate that SAS4 and SAS5 can function on a SIR1-independent silencing pathway. Furthermore, SAS4 and SAS5 contribute to silencing at HML. Double mutant analysis of silencing at HML between SAS4, SAS5 and SIR1 suggests that SAS4 and SAS5 may have similar function as SIR1 acting in establishment of silencing. Taken together, these evidence demonstrates that there exist multiple SIR-dependent pathways of silencing in yeast, which provide first genetic evidence of existence of multiple pathways of positon effects in any eukaryote.
Graduation Semester
1998
Type of Resource
text
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http://hdl.handle.net/2142/86733

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