Elucidation of a Macrophage Cell Surface Receptor With Specificity for Exogenous Aromatic Hapten-Protein Antigens and Effects on Antigen Uptake and Processing

Cherukuri, Anupama

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Description

Title

Elucidation of a Macrophage Cell Surface Receptor With Specificity for Exogenous Aromatic Hapten-Protein Antigens and Effects on Antigen Uptake and Processing

Author(s)

Cherukuri, Anupama

Issue Date

1998

Doctoral Committee Chair(s)

Voss, Edward W., Jr.

Department of Study

Microbiology

Discipline

Microbiology

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

Health Sciences, Immunology

Language

eng

Abstract

Quantitative analyses of the binding interactions demonstrated that the kinetics of antigen uptake was dependent on the extent of receptor crosslinkage at the cell surface as well as on the accessibility of the ligand groups. Determination of the ligand-binding specificity of the macrophage receptor suggested preference for aromatic ligands like phenylalanine, 2-phenyloxazolone and fluorescein. Biochemical purification and partial characterization of the putative receptor were undertaken as one of the major goals of this thesis. The experiments described herein are the first to convincingly demonstrate the existence of an aromatic hapten-binding plasma membrane protein on macrophage cells with specificity for fluorescein. Moreover, the models proposed in this thesis have helped the further elucidation of the finer principles that dictate exogenous antigen processing and presentation in macrophage cells.

Graduation Semester

1998

Type of Resource

text

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