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https://hdl.handle.net/2142/86727
Description
Title
Oxygen Regulated Genes in Salmonella Typhimurium
Author(s)
Wei, Yan
Issue Date
1997
Doctoral Committee Chair(s)
Miller, Charles G.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
Studies on oxd-2 showed that it encodes a 42-kD protein which has acetate/propionate kinase activities. The cloned oxd-2 gene, under a foreign promoter, complemented an ackA (encoding an acetate kinase) mutation. Both Oxd-2 and AckA have a similar substrate range, and Oxd-2 has higher affinity for propionate than for acetate. Transcription of oxd-2 was induced to high levels during anaerobic growth in an amino acid rich medium. The highest level was found in cells grown anaerobically in a medium containing serine as a carbon source and supplemented with aspartate, leucine, and threonine. oxd-2 expression was affected by mutations in fnr, hip (IHF), crp, lrp, ackA or pta. NMR analysis of the cultures of a wildtype strain, grown anaerobically in a medium containing threonine, aspartate, leucine and casamino acids, showed that S. typhimurium produced propionate from threonine.
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