The Roles of Peroxynitrite and Superoxide in Oxidative Damage to E. Coli
Opperman, Kay Keyer
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https://hdl.handle.net/2142/86724
Description
Title
The Roles of Peroxynitrite and Superoxide in Oxidative Damage to E. Coli
Author(s)
Opperman, Kay Keyer
Issue Date
1997
Doctoral Committee Chair(s)
Imlay, James A.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Peroxynitrite (ONOO$\sp{-}),$ a toxic biomolecule produced from the reaction of superoxide and nitric oxide (NO$\cdot)$ in the phagolysosome, may also be toxic by the same mechanism as O$\sb2{-}.$ Our in vivo results indicated that peroxynitrite has the same intracellular targets as superoxide. Of nineteen enzymes tested, only the dehydratase enzymes containing these iron-sulfur clusters were significantly affected by peroxynitrite challenge. These iron-sulfur clusters could be repaired, and both iron storage proteins and iron import seem to be necessary for efficient repair. This enzyme inactivation led to an increased intracellular free iron. However, unlike superoxide. the free iron was rapidly sequestered.
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