University of Illinois Urbana-Champaign

Investigation of Adenylate Kinases Isolated From Mesophilic and Thermophilic Members of the Archaeal Genus Methanococcus

Haney, Paul Jeffrey

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Description

Title
Investigation of Adenylate Kinases Isolated From Mesophilic and Thermophilic Members of the Archaeal Genus Methanococcus
Author(s)
Haney, Paul Jeffrey
Issue Date
1997
Doctoral Committee Chair(s)
Konisky, Jordan
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Construction of chimeric proteins between M. voltae and M. jannaschii AKs along with site specific mutational analysis confirm the importance of specific stabilizing interactions identified by sequence comparisons, and allow the physical characteristic of these proteins to be significantly altered. Simple protein engineering increased the temperature optima of the Methanococcus voltae AK from 37$\sp\circ$C to 62$\sp\circ$C and its melting point from 68$\sp\circ$C to 89$\sp\circ$C while maintaining specific activity levels and approximately 90% of its original sequence. Analysis of the chimerical proteins also point strongly to the cooperative and non-additive nature of thermal stabilizing interactions within the methanococcal AKs. These studies also indicate that the molecular mechanisms which determine enzymatic temperature optima and overall protein stability are not directly related.
Graduation Semester
1997
Type of Resource
text
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http://hdl.handle.net/2142/86722

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Dissertations and Theses - Microbiology