University of Illinois Urbana-Champaign

Integration and Excision Mechanism of Bacteroides Conjugative Transposons

Song, Bo

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Description

Title
Integration and Excision Mechanism of Bacteroides Conjugative Transposons
Author(s)
Song, Bo
Issue Date
2008
Doctoral Committee Chair(s)
Abigail Salyers
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Microbiology
Language
eng
Abstract
The third project is about the regulatory mechanism of RteB on CTnDOT. RteA and RteB are shown to be global regulators that regulate many genes on Bacteroides host. Whether RteB regulates CTnDOT and NBU1 excision directly or not is investigated in this study. Both RteB and the mutant are over-expressed in E. coli and partially purified. RteB is a soluble protein and gel shift result shows that RteB binds to 168bp upstream of rteC promoter region. However, the mutant did not bind to any probes tested. Footprint assay is required to determine the exact binding site on rteC promoter region.
Graduation Semester
2008
Type of Resource
text
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http://hdl.handle.net/2142/86704

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Dissertations and Theses - Microbiology