University of Illinois Urbana-Champaign

DNA -Binding Proteins That Facilitate Integration of Transmissible DNA Elements in Bacteroides Thetaiotaomicron

Romero-Guss, Melanie Claire

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https://hdl.handle.net/2142/86689

Description

Title
DNA -Binding Proteins That Facilitate Integration of Transmissible DNA Elements in Bacteroides Thetaiotaomicron
Author(s)
Romero-Guss, Melanie Claire
Issue Date
2006
Doctoral Committee Chair(s)
Salyers, Abigail A.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Microbiology
Language
eng
Abstract
I implemented a biochemical approach utilizing heparin column, size-exclusion and DNA-affinity chromatography, 2-D gel electrophoresis and mass fingerprinting to identify proteins that bound the attachment sites of CTnDOT (attDOT ) and NBU1 (attNI), and stimulated integration in vitro for both DNA elements. The proteins isolated and characterized include BHP, a non-specific DNA-binding protein that is most likely involved in nucleoid organization, BHP2, a homolog to BHP that probably functions in a similar capacity, and BT 1847, a specific DNA-binding protein that is a member of the AraC/Xy1S transcription regulator family. Preliminary data suggest that another protein, BT1271, works in conjunction with BT1847 to confer DNA-binding and specificity. BT1271 is annotated in the genome as the 30S ribosomal subunit S16.
Graduation Semester
2006
Type of Resource
text
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http://hdl.handle.net/2142/86689

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Dissertations and Theses - Microbiology