DNA -Protein Interactions in the CTnDOT Excisive Intasome
Dichiara, Jeanne M.
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https://hdl.handle.net/2142/86685
Description
Title
DNA -Protein Interactions in the CTnDOT Excisive Intasome
Author(s)
Dichiara, Jeanne M.
Issue Date
2006
Doctoral Committee Chair(s)
Gardner, Jeffrey F.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Microbiology
Language
eng
Abstract
The excision mechanism of CTnDOT is more complicated than any system studied to date. Excision requires multiple accessory proteins. Exc is one protein required for excision, however, its function is unknown. In an attempt to determine a role for Exc, the native wild type Exc protein was purified. These studies indicated that Exc might interact with IntDOT during excision, as determined by electrophoretic mobility shift assays. Further studies of Exc are warranted to answer the many questions that still remain about its function.
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