Engineering T Cell Receptors to Examine the Role of Affinity and *Kinetics in T Helper Cell Function
Weber, K. Scott
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https://hdl.handle.net/2142/86681
Description
Title
Engineering T Cell Receptors to Examine the Role of Affinity and *Kinetics in T Helper Cell Function
Author(s)
Weber, K. Scott
Issue Date
2005
Doctoral Committee Chair(s)
Kranz, David M.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Health Sciences, Immunology
Language
eng
Abstract
Chapter five details the engineering of a class II restricted human TCR isolated from a patient with multiple sclerosis called 3A6. Yeast display of TCRs has previously relied on the use of an antibody specific for a properly folded TCR, here various strategies were used to generate stabilized 3A6 TCRs. Helpful strategies included: alteration of error-prone PCR dNTP concentrations, increasing induction media galactose concentrations, and generation of three-domain TCR constructs (Valpha-VbetaCbeta).
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