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https://hdl.handle.net/2142/86677
Description
Title
Studies of Lipoic Acid Related Enzymes
Author(s)
Jiang, Yanfang
Issue Date
2005
Doctoral Committee Chair(s)
Cronan, John E., Jr
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Microbiology
Language
eng
Abstract
Using a similar strategy of library construction and screening, I also isolated the Vibrio harveyi acyl-ACP synthetase gene and showed that it encoded a 60.4 KDa protein that shared high homology with medium chain fatty acid-CoA ligases. I expressed the His-tagged protein at a high level in E. coli and purified it to homogeneity. To explore the physiological application of this enzyme in E. coli fatty acid metabolism, I constructed the first E. coli fabH (beta-ketoacyl-ACP synthetase III) bypass mutant in the presence of acyl-ACP synthetase and exogenous fatty acids.
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