Coordination and Catalysis of the Lambda Integrase Site -Specific Recombination Reaction
Kazmierczak, Robert Andrew
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https://hdl.handle.net/2142/86671
Description
Title
Coordination and Catalysis of the Lambda Integrase Site -Specific Recombination Reaction
Author(s)
Kazmierczak, Robert Andrew
Issue Date
2004
Doctoral Committee Chair(s)
Gardner, Jeffrey F.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
In the course of my doctoral work, I have developed quantitative assays to measure the maximal cleavage and ligation activity of Integrase. From this work, we concluded that para-Nitrophenol tyrosine analogs are optimal for quantitative in vitro measurement of Int ligation. Int could not utilize tyrosine analogs para-Cresol and dimethyl- p-phenylenediamine as ligation substrates under my reaction conditions. However, they may be useful to identify and characterize Int or other tyrosine recombinase proteins with enhanced ligation activities.
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