University of Illinois Urbana-Champaign

Molecular Profiling: Gene Expression Reveals Discrete Phases of Lens Induction and Development in Xenopus Laevis

Walter, Brian Edward

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Description

Title
Molecular Profiling: Gene Expression Reveals Discrete Phases of Lens Induction and Development in Xenopus Laevis
Author(s)
Walter, Brian Edward
Issue Date
2004
Doctoral Committee Chair(s)
Henry, Jonathan J.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Zoology
Language
eng
Abstract
"From these genes, two were examined to further characterize their roles in development and transdifferentiation. The matrix metalloproteinase Xmmp-9 was found to function during migratory and morphogenetic processes during development, and it was found to be upregulated during wound healing. Psf2 codes for a factor which functions during DNA replication as a member of the GINS heterotetramer (Kubota et al., 2003), a ""sliding clamp"" involved in DNA replication. In situ hybridization revealed that Psf2 is expressed during embryonic development in a tissue-specific manner, including the optic cup and the lens. Morpholino-mediated knockdown of Psf2 resulted in dosage-dependent phenotypes which included microphthalmia, coloboma, and abnormal lens formation. Additionally, tissue manipulation experiments in conjunction with morpholino injections demonstrated intrinsic roles for Psf2 in retinal and lens development and, secondarily, during the late phase of lens induction."
Graduation Semester
2004
Type of Resource
text
Permalink
http://hdl.handle.net/2142/86667

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