Negative Regulation of the accBC Operon in Escherichia Coli
James, Ethan Siddhartha
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https://hdl.handle.net/2142/86650
Description
Title
Negative Regulation of the accBC Operon in Escherichia Coli
Author(s)
James, Ethan Siddhartha
Issue Date
2003
Doctoral Committee Chair(s)
Cronan, John E., Jr
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
Expression of the accB gene from B. subtilis complemented E. coli AccB activity, but did not cause negative regulation of the accBC genes. A chimeric protein consisting of the amino-terminal 44 amino acids of E. coli AccB fused to the carboxyl-terminal biotinylation domain of B. subtilis AccB complemented AccB activity and caused negative regulation of the accBC operon. Complementation of ACC activity using the malonyl-CoA synthase gene (matB) from Rhizobium trifolii allowed the deletion of the accA and accB genes. Transcriptional activity of the accBC operon in this strain shows an absence of growth-rate dependent regulation.
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