University of Illinois Urbana-Champaign

The Integration and Excision Mechanisms of the Bacteroides Conjugative Transposon CTnDOT

Cheng, Qi

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https://hdl.handle.net/2142/86636

Description

Title
The Integration and Excision Mechanisms of the Bacteroides Conjugative Transposon CTnDOT
Author(s)
Cheng, Qi
Issue Date
2002
Doctoral Committee Chair(s)
Gardner, Jeffrey F.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Genetics
Language
eng
Abstract
A critical requirement for studying transposition is the availability of a defined in vitro transposition system. Using the partial-purified CTnDOT Int protein, an in vitro integration system was developed and a host factor(s) from Bacteroides cell extract was shown to be important for integration. The CTnDOT Int protein catalyzes the in vitro integration reactions by a coupling sequence mechanism similar to the in vivo reaction. Purified E. coli integration host factor (IHF) can substitute for the Bacteroides host factor(s) and significantly stimulates in vitro integration.
Graduation Semester
2002
Type of Resource
text
Permalink
http://hdl.handle.net/2142/86636

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Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Microbiology