University of Illinois Urbana-Champaign

Protein -Dna Interactions Required for Regulation of the Proline Utilization Operon in Salmonella

Gomez-Curet, Ilsa

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Description

Title
Protein -Dna Interactions Required for Regulation of the Proline Utilization Operon in Salmonella
Author(s)
Gomez-Curet, Ilsa
Issue Date
2001
Doctoral Committee Chair(s)
Stanley Maloy
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
A modification of the S&barbelow;ystematic E&barbelow;volution of L&barbelow;igands by Exponential Enrichment (SELEX) approach was used to enrich for put control DNA molecules defective in PutA binding in vitro. These experiments identified nucleotide residues that may be important for the specificity of the PutA-put control region interaction. Using the lambda Red system, the wild-type put control region in the LT2 chromosome was replaced with the mutant put control region isolated by SELEX. The effects of the mutations in vivo was analyzed by proline dehydrogenase assays and beta-galactosidase assays using a putA::MudJ fusion downstream of each of the put control region mutant. Although the mutations isolated in the put control region are defective in PutA binding in vitro, they do not show a strong defect in vivo..
Graduation Semester
2001
Type of Resource
text
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http://hdl.handle.net/2142/86630

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Dissertations and Theses - Microbiology