Assessment of Parasitism of House Fly and Stable Fly Pupae (Diptera: Muscidae) by Pteromalid (Hymenoptera: Pteromalidae) Parasitoids Using Polymerase Chain Reaction (Pcr)
Ratcliffe, Susan Twohey
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https://hdl.handle.net/2142/86481
Description
Title
Assessment of Parasitism of House Fly and Stable Fly Pupae (Diptera: Muscidae) by Pteromalid (Hymenoptera: Pteromalidae) Parasitoids Using Polymerase Chain Reaction (Pcr)
Author(s)
Ratcliffe, Susan Twohey
Issue Date
1999
Doctoral Committee Chair(s)
Weinzierl, Richard A.
Department of Study
Entomology
Discipline
Entomology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Genetics
Language
eng
Abstract
Three puparia collected in 1997 produced double bands prior to digestion of the PCR product by a restriction enzyme. The sizes of the bands corresponded to the sizes produced by amplification of the DNA of Muscidifurax and Spalangia species, possibly indicating hyperparasitism. Comparison of species composition in paired samples indicated higher levels of parasitism by Spalangia spp. in PCR samples (presumably resulting from earlier attach by Spalangia species) and higher levels of parasitism by Muscidifurax spp. in puparia from which adults were allowed to emerge presumably resulting from their ability to win the contest competition. (Abstract shortened by UMI.).
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