Molecular and Genetic Analysis of the Role of Ku in Silencing and Telomere Function in Saccharomyces Cerevisiae
Vandre, Catherine
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https://hdl.handle.net/2142/86323
Description
Title
Molecular and Genetic Analysis of the Role of Ku in Silencing and Telomere Function in Saccharomyces Cerevisiae
Author(s)
Vandre, Catherine
Issue Date
2008
Doctoral Committee Chair(s)
Belmont, Andrew S.
Department of Study
Cell and Developmental Biology
Discipline
Cell and Developmental Biology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
The binding of Ku at the telomere is an integral part of maintaining the specialized chromatin structure required for genomic stability at the telomeres. To gain further insight into the signals and complexes involved in the multiple processes contributing to normal telomere function, we performed a Synthetic Genetic Array analysis focusing on genes with roles in silencing (YKU70, SIR1, SAS2, SAS5, SET1, ASF1) and telomere length regulation (YKU70, BDF1, ANC1, RAD50, TEL1, RAD52). Double mutant analysis revealed functional relationships between genes by generating a network of 241 synthetic interactions between 144 genes at 30°C and a network of 355 synthetic lethal interactions between 146 genes at 37°C. The genetic interactions highlighted the underappreciated role of chromatin remodeling proteins in normal telomere function. Additionally, a screen for suppressors of Ku senescence at 37°C identified a role for inositol phosphate signaling in telomere length regulation.
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