Characterization of Transcriptional Components in Methanocaldococcus Jannaschii: An Integrative Study of Archaeal Basal Transcription Factors and Putative Cis Elements
Jiang, Ying
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https://hdl.handle.net/2142/86317
Description
Title
Characterization of Transcriptional Components in Methanocaldococcus Jannaschii: An Integrative Study of Archaeal Basal Transcription Factors and Putative Cis Elements
Author(s)
Jiang, Ying
Issue Date
2008
Doctoral Committee Chair(s)
Gary Olsen
Department of Study
Cell and Developmental Biology
Discipline
Cell and Developmental Biology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
To acquire further information related to basal and regulatory transcription, we used phylogenetic footprinting to investigate the putative cis elements in M. jannaschii. Regions upstream of orthologous genes from four related methanococcal genomes were collected and analyzed for conserved motifs. This method was applied to identify putative rRNA promoter, and revealed novel elements potentially involved in rRNA gene transcription. Additionally, we uncovered conserved features upstream of ribosomal protein genes, some of which carrying transcription-/translation-regulatory functions.
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