Regulation of Gene Transcription by Estrogen Receptor Alpha: The Role of Cis Elements and Trans -Acting Factors
Creekmore, Amy
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Permalink
https://hdl.handle.net/2142/86312
Description
Title
Regulation of Gene Transcription by Estrogen Receptor Alpha: The Role of Cis Elements and Trans -Acting Factors
Author(s)
Creekmore, Amy
Issue Date
2008
Doctoral Committee Chair(s)
Clayton, David F.
Nardulli, Ann M.
Department of Study
Cell and Developmental Biology
Discipline
Cell and Developmental Biology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Cell
Language
eng
Abstract
The differential recruitment of coregulatory proteins to DNA-bound ERalpha plays a critical role in mediating estrogen-responsive gene expression. We previously isolated and identified retinoblastoma-associated proteins 46 (RbAp46) and 48 (RbAp48), which are associated with chromatin remodeling, histone deacetylation, and transcription repression as proteins associated with the DNA-bound ERalpha. We have demonstrated that RbAp46 and RbAp48 interact with ERalpha in vitro and in vivo, associate with ERalpha at endogenous estrogen-responsive genes, and alter expression of endogenous ERalpha-activated and -repressed genes in MCF-7 breast cancer cells. Our findings reveal that RbAp48 limits expression of estrogen-responsive genes and that RbAp46 attenuates and enhances estrogen responsiveness in a gene-specific manner. The ability of the transacting factors RbAp46 and RbAp48 to interact with ERalpha and influence its activity reveals yet another role for these multifunctional proteins in regulating gene expression.
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