In Vivo Fluorometric Assessment of the Skin Microcirculation
Oh, Deborah Kyung
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https://hdl.handle.net/2142/85491
Description
Title
In Vivo Fluorometric Assessment of the Skin Microcirculation
Author(s)
Oh, Deborah Kyung
Issue Date
1999
Doctoral Committee Chair(s)
Richard L. Magin
Department of Study
Biophysics and Computational Biology
Discipline
Biophysics and Computational Biology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Engineering, Biomedical
Language
eng
Abstract
A tissue uptake model was developed to describe the fluorescein dermofluorometer signal in terms of the local blood flow and capillary permeability. The fluorescein signal is characterized by a wash-in time constant dependent on both the blood flow and permeability. Under flow-limited conditions the wash-in time constant is inversely proportional to the blood flow only. Significant differences in the fluorescein wash-in time constant were measured between diabetic and non-diabetic subjects (p < 0.10) and between diabetic patients with chronic foot ulcers and those without ulcers (p < 0.01). A new technique, dual dye dermofluorometry, which measures the simultaneous signals from an intravascular tracer, indocyanine green, and a diffusible tracer, sodium fluorescein, was developed. The indocyanine green signal is described by a physiologically-based pharmacokinetic model with a wash-in time constant inversely proportional to the blood flow. The extravascular fluorescein signal is extracted from the observed signal by subtracting a scaled version of the indocyanine green signal. Indocyanine green wash-in time constants were significantly different between patients whose foot ulcers led to amputation relative to those whose foot ulcers healed without surgery (p < 0.10) and between patients with past foot ulcers relative to patients with no history of foot ulcers (p < 0.05). It was determined that while the flow-limited assumption for fluorescein was valid in healthy rat skin, it was not valid in diabetic subjects.
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