Fluorescent Tools for Watching Conformational Changes of Biological Molecules
Toprak, Erdal
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Permalink
https://hdl.handle.net/2142/85466
Description
Title
Fluorescent Tools for Watching Conformational Changes of Biological Molecules
Author(s)
Toprak, Erdal
Issue Date
2007
Doctoral Committee Chair(s)
Selvin, Paul R.
Department of Study
Biophysics and Computational Biology
Discipline
Biophysics and Computational Biology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biophysics, General
Language
eng
Abstract
Finally, we used a single molecule fluorescence quenching assay to study the timing of the neck linker conformational change that occurs as kinesin walks processively along a microtubule. We observed repetitive, alternating cycles of fluorescence and quenching as individual kinesin motors walked along microtubules. By monitoring the position of the kinesin during these fluorescence cycles, we were able to conclude that one cycle of quenching/unquenching occurs with each 8 nm step, and that each step corresponds to an ATP-induced separation of neck linkers.
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