Structural and Biochemical Studies of Nisin *Biosynthesis and Autoimmunity
Yu, John-Paul Jaewoon
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https://hdl.handle.net/2142/85455
Description
Title
Structural and Biochemical Studies of Nisin *Biosynthesis and Autoimmunity
Author(s)
Yu, John-Paul Jaewoon
Issue Date
2006
Doctoral Committee Chair(s)
Nair, Satish K.
Department of Study
Biophysics and Computational Biology
Discipline
Biophysics and Computational Biology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biophysics, General
Language
eng
Abstract
The lantibiotic nisin is a 34-residue bacteriocin produced by the Gram-positive bacteria Lactococcus lactis spp. It is first ribosomally synthesized as an inactive precursor which is then post-translationally modified into its mature biologically active form. Described herein is a two-part study investigating (1) the structural basis of how the characteristic nisin lanthionine rings are installed by the enzyme NisC and (2) how Lactococcus lactis spp., despite producing an effective bacteriocin like nisin, is resistant to the toxic effects of nisin. The results described here together provide the first structural insights into the biosynthesis of nisin as well as insights into how L. lactis demonstrates autoimmunity to the lantibiotic nisin.
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