Junction Specificity and Transmembrane Linkages of Integrins

McDonald, Kevin Alan

Permalink

https://hdl.handle.net/2142/85391 Copy

Description

Title

Junction Specificity and Transmembrane Linkages of Integrins

Author(s)

McDonald, Kevin Alan

Issue Date

1997

Doctoral Committee Chair(s)

Horwitz, Alan F.

Department of Study

Biology

Discipline

Biology

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

Biology, Molecular

Language

eng

Abstract

We have also isolated a monoclonal antibody, P4B2, which localizes to multiple adhesion junctions, namely, a subset of focal adhesions, the Z-disc of muscle, and neuromuscular junctions. Immunopurification of the antigen to this antibody from chicken brain tissue yielded a complex of three prominent proteins with mobilities of 36, 30 and 18 kD. Amino acid sequencing of the purified proteins identified the 36 kD protein as GAPDH. The other 2 protein bands were heterogeneous, containing proteins found in the synaptic vesicle fusion core complex. Immunolocalization of P4B2 antigen in developing cultured muscle cells showed that the antigen is incorporated into Z-lines soon after the sarcomeric architecture was positive for $\alpha$-actinin. Together, the data indicate the P4B2 antigen is part of a unique GAPDH containing protein complex that may be involved in reinforcement of established cytoskeletal structures.

Graduation Semester

1997

Type of Resource

text

Permalink

http://hdl.handle.net/2142/85391

Owning Collections